Selecting The Right ELISA And Optimization

The enzyme-linked immunosorbent assay (ELISA), was first described in 1971 by Engvall and Perlmann. During that time the common assays used radioactively labeled antibodies and antigens and so ELISA was a welcome change. Initially, it was used for the detection of immunoglobulin G.

The observation in the 1960s that antibodies or antigens could be adsorbed onto hard surfaces and still participate in high-affinity binding led to the development of ELISA. You can also buy the human MAG elisa kit easily from various sources.

Today the term ELISA refers to a variety of immunoassays, some of which involve enzymatic reactions. Antibodies play a key role in determining the sensitivity and specificity of the assay. They are widely used in several fields of biomedical research and discovery, and for diagnostic purposes.

There are various ways to perform an ELISA and the use of each depends on several factors. Determining the right ELISA for a particular research question definitely depends on the desired sensitivity and what is actually being detected.

In addition, before choosing an ELISA technique, the user should know the different types of ELISA, with an emphasis on the advantages and disadvantages of each. The first is what format to use; Direct, indirect, sandwich, competitive or inhibitory ELISA. The formats differ in the way the target antigen is captured and detected.

Choice of ELISA technique

There are many factors that determine which ELISA technique is appropriate. For example, detection of small molecules such as haptens requires competitive ELISA, whereas large proteins with multiple epitopes such as e.g. Cytokines, sandwich ELISA are required.

If only one antibody to the desired antigen is available, direct or competitive ELISA can be used. Sandwich or competitive ELISA can be used to detect or quantify analytes. Direct or indirect ELISA is the best for measuring immune response.